Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant

Catalog #

90145-A

$130 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Recombinant GM-CSF is a disulfide-linked monomer protein, consisting of 127 amino acids and migrates as an approximately 15 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in E. coli.

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Synonyms

GM-CSF, CSF2, Granulocyte Macrophage-Colony Stimulating Factor, Colony Stimulating Factor-2, Molgramostin, Sargramostim, CSF

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.

15 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

18–144

Biological Activity

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Genbank #

P04141

UniProt #

P04141

Background

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the N-terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.

References

1. Hiasa, M., et al. Blood November 12, 2009 vol. 114 (20): 4517-4526 .
2. Zhu, S.M., et al. J. Exp. Med. 206 (10): 2141-2149.
3. Chin, P.Y., et al. Hum. Reprod. (2009) 24 (12): 2997-3009.

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Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant

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