GFRAL, Fc Fusion, Avi-Tag, Biotin-Labeled Recombinant

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Catalog #

101013

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Description

Recombinant human GFRAL (GDNF family receptor alpha like) encompassing amino acids 19-351(end). This protein is fused in C-terminal with the Fc fragment of human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Synonyms

C6orf144, GRAL, UNQ9356, bA360D14.1, GDNF family receptor alpha like

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Biotin-Avidin Pulldown

GDF15: GFRAL-Biotin Binding Assay

GDF15: GFRAL[Biotinylated]inhibition curve using a colorimetric assay (#78807). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

GFRAL (19-351(end)-Fc (lgG1)-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

67 kDa + glycans

Amino Acids

19-351(end)

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_207410.2

UniProt #

Q6UXV0

Tag(s)

C-terminal Fc-Avi-Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

An ELISA binding assay was performed with GFRAL and GDF15. GDF15 was coated onto an assay plate overnight at 4 µg/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of GFRAL were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Absorbance was measured on a plate reader at 450 nm.

A neutralization binding assay was performed with Anti-GFRAL Antibody (BPS Bioscience #101351) to test its ability to inhibit the binding of GFRAL (BPS Bioscience #101013) and GDF15. GDF15 was coated onto an assay plate overnight at 4 µg/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of anti-GFRAL were added (0 to 200 nM) and allowed to preincubate for 30 minutes at room temperature before 1.5 ng/µl of GFRAL was added and continued to incubate for 1 hour. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Absorbance was measured on a plate reader at 450 nm

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only

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Datasheets/Protocols

101013 Lot 220505

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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GFRAL, Fc Fusion, Avi-Tag, Biotin-Labeled Recombinant

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