GFP, His-tag Recombinant
Catalog #
50277
$425
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Description
Recombinant Jellyfish (Aequorea victoria) green fluorescent protein, full length with C-terminal His-tag, MW= 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.
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Synonyms
GFP, Green Fluorescent Protein GFP
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Product Data Gallery
Product Info
Storage and Usage
Citations
Species
Jellyfish
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
MW
29.9 kDa
Amino Acids
full length
UniProt #
P42212
Background
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
References
1. Chudakov DM, et al. Physiol Rev. 2010
Jul;90(3):1103-63.
2. Gerdes HH, Kaether C. FEBS Lett. 1996 Jun 24;389(1):44-7.
2. Gerdes HH, Kaether C. FEBS Lett. 1996 Jun 24;389(1):44-7.