GFP, His-tag Recombinant

Catalog #
50277
$425 *
Size: 200 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant Jellyfish (Aequorea victoria) green fluorescent protein, full length with C-terminal His-tag, MW= 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.

Synonyms
GFP, Green Fluorescent Protein GFP
Product Info
Storage and Usage
Citations
Species
Jellyfish
Host Species/Expression System
E. coli
Purity

≥90%

Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
MW
29.9 kDa
Amino Acids
full length
UniProt #
P42212
Tag(s)
C-terminal His-tag
Background
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
References
1. Chudakov DM, et al. Physiol Rev. 2010 Jul;90(3):1103-63.
2. Gerdes HH, Kaether C. FEBS Lett. 1996 Jun 24;389(1):44-7.