GAS Reporter (Luc)-HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)

Catalog #

79041

$1,810 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of IFN-signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Gas Cell line, GAS reporter cell line, GAS Hela cell line, STAT1, Just another kinase, JAK, INFγ, Signal transducer and activator of transcription 1, Interferon gamma cell line, JAK pathway bioassay

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Product Data Gallery

IFNγ dose response in GAS reporter (Luc)-HeLa cells

GAS reporter activity in response to IFNα

Product Info

Storage and Usage

Citations

Host Cell Line

HeLa

Supplied As

Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

• IFNγ (PeproTech #300-02)

• IFNα (PBL Assay Science #11100-1)

• Assay medium: Thaw Medium 1 (BPS Bioscience, #60187) or

• MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS, 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

• 96-well tissue culture treated white clear-bottom assay plate (Corning #3610)

• One-Step luciferase assay system (BPS Bioscience #60690)

• Luminometer

UniProt #

P23458, O60674, P01579, P42224

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

References

1. Decker T, Kovarik P, Meinke A. (1997) GAS elements: a few nucleotides with a majorimpact on cytokine-induced gene expression. J Interferon Cytokine Res. 17(3): 121-34.

2. Darnell J, Kerr IM, Stark GR. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Complete Growth Medium: MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin and 800 µg/ml of Geneticin

Cells should be grown at 37^oC with 5% CO₂ using complete growth medium.

Instructions for Use

See data sheet for detailed culturing and assay protocol

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only