GAS Reporter (Luc) – HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)

Catalog #: 79041
Size: 2 vials
$1,295
Description:

The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

Materials Required But Not Provided:

• IFNγ (PeproTech #300-02)

• IFNα (PBL Assay Science #11100-1)

• Assay medium: Thaw Medium 1 (BPS Bioscience, #60187) or

• MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS, 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

• 96-well tissue culture treated white clear-bottom assay plate (Corning #3610)

• One-Step luciferase assay system (BPS Bioscience #60690)

• Luminometer

Synonym(s):
Gas Cell line, GAS reporter cell line, GAS Hela cell line, STAT1, Just another kinase, JAK, INFγ, Signal transducer and activator of transcription 1, Interferon gamma cell line, JAK pathway bioassay
Supplied As:
Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO
Host Cell line:
HeLa
Mycoplasma Testing:

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Growth Media:

Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS, 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

Complete Growth Medium: Thaw Medium 1 (BPS Bioscience #60187) and 800 µg/ml of Geneticin (Life Technologies #11811031).

Cells should be grown at 37oC with 5% CO2 using complete growth medium.

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Application(s):

•Monitor interferon gamma-induced signal transduction pathways.

•Screen for activators or inhibitors of JAK/STAT1 signaling pathway using the GAS Reporter Cell Line.

Reference(s):

1. Decker T, Kovarik P, Meinke A. (1997) GAS elements: a few nucleotides with a majorimpact on cytokine-induced gene expression. J Interferon Cytokine Res. 17(3): 121-34.

2. Darnell J, Kerr IM, Stark GR. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421.

Notes:

License Disclosure:

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS Bioscience does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79041_Lot_180315_1.pdf