G9a Homogeneous Assay Kit

Catalog #

52051

$1,125 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The G9a Homogeneous Assay Kit is designed to measure the activity of G9a (also known as EHMT2 or KMT1C) for screening and profiling applications, using AlphaLISA^® technology. The key to the G9a Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing G9a enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against G9a? Check out our Methyltransferase Screening Services.

This product has been cited 1 time.

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Synonyms

G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

AlphaLISA®

Supplied As

Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, methylation assay buffer, and purified G9a for 384 enzyme reactions.

Materials Required But Not Supplied

DTT (Dithiothreitol), 0.5M (Sigma, Cat. # D0632)
AlphaLISA anti-mIgG acceptor beads, 5 mg
AlphaLISA anti-mIgG acceptor beads, 5 mg/ml (PerkinElmer #AL105C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate -384 (PerkinElmer #6007290)
AlphaScreen microplate reader

Format

Catalog Number	Components	Amount	Storage
51001	G9a enzyme	8 µg	-80°C	Avoid freeze/ thaw cycles!
52120	100 μM S-adenosylmethionine	2 x 250 µl	-80°C
52140E	Primary antibody 5	2 x 12.5 µl	-80°C
	Biotinylated histone H3 peptide substrate (G9α)	10 µl	-80°C
	4x G9a assay buffer 1A (add DTT before experiment)	3 ml	-20°C
	4x Detection buffer 1	2 ml	-20°C

UniProt #

Q96KQ7

Background

G9a is a histone methyltransferase that exhibits methylation activity toward H3-K9.

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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G9a Homogeneous Assay Kit

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