FTO Chemiluminescent Assay Kit

Catalog #

79344

$765 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against FTO? Check out our Demethylase Screening Services or DNA Replication and Repair Screening Services.

This product has been cited 5 times.

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Synonyms

ALKBH9, BMIQ14

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Product Data Gallery

Product Info

Storage and Usage

Citations5

Assay Kit Format

Chemiluminescent

Supplied As

The FTO Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate precoated with methylated FTO substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified FTO for 96 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
79306	FTO	100 µg	-80°C	Avoid freeze/ thaw cycles!
52140Z4	Primary antibody 29	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
	4x FTO assay buffer	3 x 1 ml	-80°C
79556	Blocking buffer 1	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	RT
79670	ELISA ECL substrate B (brown bottle)	6 ml	RT
	White 8-well strip plate module precoated with RNA substrate*	1	+4°C

*Make sure you are working in RNAse-free conditions

UniProt #

Q9C0B1

Background

The methylation of internal adenosines at the N⁶ position (m⁶A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer’s disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m⁶A demethylase identified.

References

Zhou Y., et al. J Biomed Sci. 2017; 24(1):65.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only