Foxp3 Luciferase Reporter Jurkat Cell Line

Catalog #

60628

$7,645 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The forkhead box P3 (Foxp3, also known as scurfin) Luciferase Reporter Jurkat cell line is designed for monitoring the transcriptional activity of the Foxp3 promoter and testing inhibitors that target Foxp3-inducing signaling pathways. It contains a firefly luciferase gene under the control of a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

This reporter cell line has been tested and validated using phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin. It can be used to measure T cell activation mediated by Dynabeads T-Activator CD3/CD28.

Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line

Figure 1: Illustration of the Foxp3 promoter region with representative transcription factor binding sites and enhancer regions, and illustration of the cell-based assay principle (created with BioRender.com).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of Foxp3 signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Foxp3, Foxp3 cell line, scurfin cell line, foxo

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Product Data Gallery

Activation of Foxp3 Luciferase Reporter Jurkat cell by PMA and Ionomycin

Foxp3 Reporter Jurkat response to TCR activator CD3/CD28 Dynabeads™

Inhibition of PMA/Ionomycin-induced Foxp3 Luciferase Reporter activity by Cyclosporin A

Product Info

Storage and Usage

Citations1

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2B	BPS Bioscience #79530

Materials Required for Cellular Assay

Name	Ordering Information
Assay Medium 2E	BPS Bioscience #78826
Phorbol 12-myristate 13-acetate (PMA)	LC Laboratories #P1680
Ionomycin	Sigma #I3909
Dynabeads™ Human T-Activator CD3/CD28	Thermo Fisher #11131D
Cyclosporin A	Sigma #30024
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer
96-well tissue culture-treated white clear-bottom assay plate

UniProt #

Q9BZS1

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Foxp3, belonging to the forkhead family, is a master transcriptional regulator that is expressed exclusively in regulatory T cells, a subset of CD4⁺ T cells that downregulate immune responses. Regulation of Foxp3 is critical for maintaining immunological tolerance, and over-expression of Foxp3 is known to suppress effector T cell activation. The promoter of the gene encoding Foxp3 contains sequence binding elements recognized by transcription factors that coordinate the expression of Foxp3, including CREB (CAMP Response Element Binding), GAS (Gamma interferon activation site) and NFAT (nuclear factors of activated T cells) binding elements.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2B (BPS Bioscience #79530): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin and 1 mg/ml G418.

Media Required for Functional Cellular Assay

Assay Medium 2E (BPS Bioscience #78826): RPMI 1640 medium supplemented with 0.5% FBS, 1% Penicillin/Streptomycin.

Applications

Screen for compounds that affect transcription factors that regulate the expression of Foxp3 in a cellular context.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only