Sirtuin 6 Fluorogenic Assay Kit

Catalog #

50022

$445 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic SIRT6 Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against Sirtuin 6? Check out our Deacetylase/Sirtuin Screening Services.

This product has been cited 4 times.

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Synonyms

SIRT6, Sirtuin 6, SIRT, SIRT 6, Fluorogenic Sirtuin 6 Assay Kit

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Product Data Gallery

Product Info

Storage and Usage

Citations4

Assay Kit Format

Fluorogenic

Supplied As

Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control,

Materials Required But Not Supplied

BSA (bovine serum albumin) (1 mg/ml)
Fluorescent microplate reader

Format

Catalog Number	Reagent	Amount	Storage
50017	SIRT6 human recombinant enzyme	100 µg	-80°C	Avoid freeze/ thaw cycles!
50866	Fluorogenic SIRT6 substrate (5 mM)	50 µl	-80°C
	Nicotinamide Adenine Dinucleotide (NAD+) (50 mM)	50 µl	-80°C
	Nicotinamide (10 mM)	500 µl	-80°C
	2x SIRT Developer (contains 2 mM Nicotinamide)	6 ml	-80°C
50090	SIRT assay buffer	10 ml	-20°C
79685	black, low binding NUNC black microtiter plate	1 plate	Room Temp.

UniProt #

Q8N6T7

Background

Sirtuins are NAD+ dependent class III histone deacetylases that regulate important biological processes including metabolism and aging. In human, there are seven isoforms of Sirtuins, SIRT1 to SIRT7. Four of the isoforms, SIRT4 to SIRT7, have no detectable or very weak deacetylase activity. Sirtuin 6 (SIRT6) is shown to be a potent defatty-acylase of lysine residues.

References

1. Hu, J. et al., Org Miomol Chem, 2013. 11(32):5213-5216.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only