SIRT5 (Sirtuin5) Fluorogenic Assay Kit

Catalog #: 50085
Size: 96 reactions
$430
Description:
The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
UniProt
Q9NXA8
Synonym(s):
sirt5, sirtuin 5
Supplied As:
The Fluorogenic SIRT5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
1.J. Du et al. (2011) Science. 334: 806.
2. A. Madsen et al. (2012) J. Med. Chem 55: 5582.
3. F. Fisher et al. (2012) Plos One. 7: 1.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50085.pdf