SIRT1 (Sirtuin1) Fluorogenic Assay Kit

Catalog #
50081
$445 *
Size: 96 reactions
Qty
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Description

The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the HDAC fluorometric substrate (HDAC substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against SIRT1 (Sirtuin1)? Check out our Deacetylase/Sirtuin Screening Services.

This product has been cited 14 times.

Synonyms
sirt1, Sir2, Sir2a
Product Info
Storage and Usage
Citations14
Assay Kit Format
Fluorogenic
Supplied As
Kit comes in a convenient 96-well format, with reagents, purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control necessary for 100 fluorescent SIRT1 (Sir2) activity measurements.
Format

 

Catalog
Number

Reagent

Amount

Storage
50012 SIRT1 human recombinant enzyme 50 µg -80°C



Avoid freeze/
thaw cycles!
50032 Fluorogenic HDAC substrate (5 mM) 50 µl -80°C
  Nicotinamide Adenine Dinucleotide (NAD+)
(50 mM)

50 µl

-80°C
  Nicotinamide (10 mM) 500 µl -80°C
50089 2x SIRT Developer
(contains 2 mM Nicotinamide)

6 ml

-80°C
50090 SIRT assay buffer 10 ml -20°C
79685 Black, low binding NUNC black microtiter plate
1 plate
Room
Temp.
UniProt #
Q96EB6
References

1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.