HDAC8 Fluorogenic Assay Kit

Catalog #
50068
$445 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The Fluorogenic HDAC 8 Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First, the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against HDAC8? Check out our Deacetylase/Sirtuin Screening Services.

This product has been cited 15 times.

Synonyms
histone deacetylase, HDAC, HDAC-8
Product Info
Storage and Usage
Citations15
Assay Kit Format
Fluorogenic
Format
Catalog # Component Amount Storage
50008 HDAC8 human recombinant enzyme* 2 µg  -80°C Avoid freeze/ thaw cycles
50040 Fluorogenic HDAC substrate class 2A (5 mM) 25 µl -80°C
50030 2x HDAC Developer (contains Trichostatin A)
(2 µM) 
6 ml -80°C
  Trichostatin A (1 mM) in DMSO 100 µl -20°C
50031 HDAC Assay Buffer 10 ml -20°C
79685 black, low binding NUNC black microtiter plate 1 plate Room Temp

*A large excess of enzyme is supplied.  

UniProt #
Q9BY41
Background
HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.
References

1. Vijayakumar, B., et al. Bioinformation 2011; 7(3): 134-41.
2. Oehme, I., et al. Expert Opin Investig Drugs . 2009 Nov; 18(11): 1605-17.