HDAC6 Fluorogenic Assay Kit
The Fluorogenic HDAC6 Assay Kit is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. In addition, the kit includes purified HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
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This product has been cited 22 times.
0.1% solution (1 mg/ml) of bovine serum albumin (BSA) in water
Fluorimeter capable of excitation at 350-380 nm and detection at 440-460 nm.
Adjustable micropipettor and sterile tips
Rotating or rocker platform
96 Reactions
Catalog # | Component | Amount | Storage | |
50006 | HDAC6 human recombinant enzyme | 9 µg | -80°C |
Avoid |
50037 | Fluorogenic HDAC substrate 3 (5 mM) | 25 µl | -80°C | |
50030 | 2x HDAC Developer (contains Trichostatin A) (2 µM) |
6 ml | -80°C | |
Trichostatin A in DMSO (200 µM) |
100 µl | -20°C | ||
50031 | HDAC Assay Buffer | 10 ml | -20°C | |
79685 | Black, low binding NUNC black microtiter plate | 1 | Room Temp |
384 Reactions
Catalog # | Component | Amount | Storage | |
50006 | HDAC6 human recombinant enzyme | 9 µg | -80°C |
Avoid |
50037 | Fluorogenic HDAC substrate 3 (5 mM) | 50 µl | -80°C | |
50030 | 2x HDAC Developer (contains Trichostatin A) (2 µM) |
2 x 6 ml | -80°C | |
50031 | HDAC Assay Buffer | 10 ml | -20°C | |
79961 | 384-well black, low binding microtiter plate | 1 | Room Temp |
1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.
2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar;6(3): 238-243.