Fluorogenic HDAC substrate 3

Catalog #: 50037
Size: 250 nmole
$230
Description:
A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040.
Supplied As:
5 mM DMSO solution (50 µl)
Format:
DMSO solution
Instructions for use:
Materials :
HDAC Assay buffer (BPS catalog #50031)
HDAC Assay developer (BPS catalog #50030)
HDAC Substrate 3 (BPS catalog #50037)
HDAC1 (0.33 µg/µl) (BPS catalog #50051) or other class1/2b HDAC enzyme

Step 1 :
Add the following reagents to a low binding NUNC black plate (VWR catalog #62408-936):
HDAC Assay Buffer, 38 µl
1 mg/ml BSA, 5 µl
200 µM HDAC substrate 3, 5 µl
HDAC1, 2 µl
Incubate at 37°C for 30 min.

Step 2 :
Stop the reaction by adding 50 µl of HDAC assay developer (undiluted) and incubate the plate at room temperature for 15 min.

Step 3 :
Read samples in a microtiter-plate reading fluorimeter capable of excitation at 350-380 nm and emission at 440-460 nm. “Blank” value (no enzyme negative control) is subtracted from all other values.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed

Application(s):
Study enzyme kinetics, and screen small molecule HDAC inhibitors for drug discovery and HTS applications.
Warning(s):
Protect from direct exposure to light. Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Substrate
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50037_Lot110124.pdf