Fluorogenic HDAC class 2a substrate

Catalog #

50040

$265 *

Size: 250 nmole

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7, and 9). It also works well for HDACs 8 and 11. This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC class 2a substrate 2 (green), BPS catalog #50042. For HDACs 1-3 ,6, and 10, we recommend substrate #50037.

This product has been cited 18 times.

Product Info

Storage and Usage

Citations18

Supplied As

5 mM DMSO solution (50 µl)

Format

DMSO solution

References

1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed

Instructions for Use

Materials :
HDAC Assay buffer (BPS catalog #50031)
HDAC Assay developer (BPS catalog #50030)
HDAC Class 2A Substrate (BPS catalog #50040)
HDAC4 (0.2 ng/µl) (BPS catalog #50004) or other class2a HDAC enzyme

Step 1 :
Add the following reagents to a low binding NUNC black plate (VWR catalog #62408-936):
HDAC Assay Buffer, 35 µl
1 mg/ml BSA, 5 µl
200 µM HDAC 2A substrate, 5 µl
HDAC4, 5 µl
Incubate at 37°C for 30 min.

Step 2 :
Stop the reaction by adding 50 µl of HDAC assay developer (undiluted) and incubate the plate at room temperature for 15 min.

Step 3 :
Read samples in a microtiter-plate reading fluorimeter capable of excitation at 350-380 nm and emission at 440-460 nm. “Blank” value (no enzyme negative control) is subtracted from all other values.

Applications

Study enzyme kinetics, and screen small molecule inhibitors of HDAC class2a for drug discovery and HTS applications.

Shipping Temperature

-20°C or-80°C

Warnings

Protect from direct exposure to light. Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Fluorogenic HDAC class 2a substrate

PRODUCTS

SERVICES

RESOURCES

COMPANY