GCN5 Fluorogenic Assay Kit

Catalog #: 50091
Size: 96 reactions
$450
Description:
The Fluorogenic GCN5 Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of GCN5 (also known as KAT2A). The Fluorogenic GCN5 Assay Kit is based on the transfer of an acetylgroup from acetyl CoA to a peptide substrate. After incubation with acetyl CoA and the substrate, the GCN5 enzyme generates acetylated H3 peptide and CoASH. No time-consuming washing steps are required. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360 nm and emission = 460 nm.
UniProt
Q92830
Synonym(s):
HAT, histone acetyltransferase, GCN5, KAT2A, GCNL2, PCAF-β
Supplied As:
The Fluorogenic GCN5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HAT activity measurements. In addition, the kit includes purified GCN5 for use as a positive control.
Contraindications:
DTT, β-mercaptoethanol, or other reducing reagents, DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Acetyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50091.pdf