DPP8 Assay Kit

Catalog #
80208
$515 *
Size: 96 reactions
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Description

The Fluorogenic DPP8 Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and profiling applications. The key to the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

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This product has been cited 6 times.

Synonyms
Dipeptidylpeptidase 8, MSTP141, DPP
Product Info
Storage and Usage
Citations6
Assay Kit Format
Fluorogenic
Supplied As
Supplied in a convenient 96-well format, with purified DPP8 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.
Format
Catalog
Number
Component Amount Storage
80080 DPP8 human recombinant enzyme 5 µg  -80°C Avoid
freeze/
thaw
cycles
80300 DPP assay buffer 10 ml -20°C
80305 Fluorogenic DPP substrate 1 in DMSO
(0.5mM)
100 µl -80°C
AMC Fluorescent standard (50 µM) 500 µl -20°C
79685 black, low binding NUNC black microtiter plate 1 plate Room Temp.
UniProt #
Q6V1X1
Background
Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP8 shares homology and substrate recognition patterns with DPP4 and like DPP4, is thought to play a role in T-cell activation and immune function.
References

1. Lee, H.J. et al., J. Biol. Chem. 281 (50), 38653-38662 (2006).
2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460 (2006).