FGFR2, GST-tag Recombinant

Catalog #

40211

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human FGFR2 (GenBank Accession No. NM_000141), a.a. 399-end with N-terminal GST-tag, MW= 74 kDa, expressed in a baculovirus expression system.

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Synonyms

FGFR2, Fibroblast Growth Factor Receptor 2, BEK, Keratinocyte Growth Factor Receptor, KGFR, JWS, CD332, FGFR-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥73%

Format

Aqueous buffer solution.

Formulation

25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

74 kDa

Amino Acids

399–end

Specific Activity

≥150 pmol/min/μg.

Genbank #

NM_000141

UniProt #

P21802

Tag(s)

N-terminal GST-tag

References

1. Holtrich, U. et al., Proc. Nat. Acad. Sci. (1991); 88:10411-10415.

2. Roidl, A. et al., Clin Cancer Res. (2009 Mar 15); 15(6):2058-66.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 2 mM DTT using Poly-(Glu:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40211.pdf

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