FBXL10 (KDM2B, JHDM1B), FLAG-tag

Catalog #: 50120
Size: 20 µg
$460
Species: Human
Host Species: Sf9 insect cells
MW: 75 kDa
Genbank #: NM_032590
Tag(s): C-terminal FLAG-tag
a.a: 1–650
Description:
Human F-Box Protein 10 (FBXL10, also known as KDM2B, JHDM1B, CXXC2, and Histone-H3 K36 demethylase 1B), GenBank Accession No. NM_032590, a.a. 1-650, with C-terminal FLAG-tag, MW= 75 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
Q8NHM5
Synonym(s):
F-Box Protein 10, FBXL10, KDM2B, JHDM1B, CXXC2, Histone-H3 K36 demethylase 1B
Purity:
≥83% by HPLC
Assay Conditions:
Enzyme was incubated with 0.4 μM substrate in HEPES-containing JMJ assay buffer SA and SA- (without KG) in a 10-μl reaction for 180 min at 30˚C. 5 μl of Anti-Rabbit AlphaLISA® acceptor beads (1:250) and 5 μl of rabbit polyclonal antibody (1:200) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 μl) and mixture was further incubated for 10 min at RT before Acounts were measured.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22.
2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90. 3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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