EZH2 / EED / SUZ12, His-Tag, FLAG-Tag Recombinant

Catalog #

51003

$695 *

Size: 50 µg

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Description

Recombinant human EZH2 (Histone-lysine N-methyltransferase) in complex with EED (Embryonic ectoderm development protein) and SUZ (Suppressor of zeste).

EZH2, full length encompassing amino acids 2-751 (end), contains an N-terminal His-tag (6xHis). Polycomb protein EED, full length encompassing amino acids 2-441(end), contains an N-terminal FLAG-tag. Polycomb protein SUZ12 full length encompassing amino acids 2-739 (end), also contains an N-terminal His-tag (6xHis). The complex was affinity purified using the FLAG-tag of EED.

This product has been cited 21 times.

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Synonyms

EZH2: ENX-1, ENX1, KMT6, KMT6A, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, WVS, WVS2, EZH2b EED: HEED, COGIS, WAIT1, WD protein associating with integrin cytoplasmic tails 1 SUZ12: CHET9, Chromatin precipitated E2F target 9 protein, JJ

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

EZH2/EED/SUZ12 Activity

Product Info

Storage and Usage

Citations21

Species

Human

Construct

EZH2 (His-2-751(end)) / EED (FLAG-2-441(end)) / SUZ12 (His-1-739(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

EZH2: 87 kDa; EED: 51 kDa; SUZ12: 88 kDa

Amino Acids

EZH2: 2-751(end); EED: 2-441(end); SUZ12: 2-739(end)

Genbank #

EZH2: NM_004456; EED: NM_003797; SUZ12: NM_015355

UniProt #

EZH2: Q15910; EED: O75530; SUZ12: Q15022

Tag(s)

N-terminal His-tag (EZH2, SUZ12), N-terminal FLAG-tag (EED)

Background

Polycomb-group family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2/EED/SUZ complex methylates Lys-9 and Lys-27 of histone H3, leading to transcriptional repression of the affected target genes. The complex recognizes Lys-26 trimethylated histone H1, inhibiting PRC2 complex methyltransferase activity, whereas H3 Lys-27 recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was performed using the EZH2 Chemiluminescent Assay Kit (BPS Bioscience #52009L) with EZH2 titrated from 1000 ng/rxn – 0.97 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

51003 Lot 210615

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EZH2 / EED / SUZ12, His-Tag, FLAG-Tag Recombinant

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