EZH2 (Y641N) /EED/SUZ12/RbAp48/AEBP2, His-Tag, FLAG-Tag Recombinant
Mutant version of the EZH2 5-member complex (BPS Bioscience #51004), with a Tyr-to-Asn mutation on amino acid 646 of the EZH2 protein. Complex of human EZH2 (enhancer of zeste homolog 2), amino acids 2-end, Y646N*, with N-terminal His-tag, human EED (embryonic ectoderm development), amino acids 2-441 (end) with N-terminal FLAG-tag, human SUZ12, amino acids 2-503 (end) with N-terminal His-tag, human AEBP2 (adipocyte enhancer-binding protein), amino acids 2-739 (end) with N-terminal His-tag, and human RbAp48 (retinoblastoma binding protein 4), amino acids 2-end with N-terminal His-tag, co-expressed in a baculovirus expression system. The complex was affinity purified.
This product has been cited 1 time.
≥90%
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable FLAG peptide, and 20% glycerol
EZH2 (enhancer of zeste homolog 2) is the catalytic subunit of the PRC2 (polycomb repressive complex 2)/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. It is abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).
Application Reference:
Morin, R.D., et al. Nat Genet. 2010 February; 42(2): 181–185.