EZH2 (Y641N) /EED/SUZ12/RbAp48/AEBP2, His-Tag, FLAG-Tag Recombinant

Catalog #
51028
$385 *
Size: 20 µg
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Description

Mutant version of the EZH2 5-member complex (BPS Bioscience #51004), with a Tyr-to-Asn mutation on amino acid 646 of the EZH2 protein. Complex of human EZH2 (enhancer of zeste homolog 2), amino acids 2-end, Y646N*, with N-terminal His-tag, human EED (embryonic ectoderm development), amino acids 2-441 (end) with N-terminal FLAG-tag, human SUZ12, amino acids 2-503 (end) with N-terminal His-tag, human AEBP2 (adipocyte enhancer-binding protein), amino acids 2-739 (end) with N-terminal His-tag, and human RbAp48 (retinoblastoma binding protein 4), amino acids 2-end with N-terminal His-tag, co-expressed in a baculovirus expression system. The complex was affinity purified.

This product has been cited 1 time.

Synonyms
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH2 (Y646N), EZH, Retinoblastoma Binding Protein 4 (RBBP4)
Product Info
Storage and Usage
Citations1
Species
Human
Construct
EZH2 (Y641N) (His-2-746(end)) / EED (FLAG-2-441(end)) / SUZ12 (His-1-739(end)) / RbAp48 (His-1-503(end)) / AEBP2 (His-2-425(end))
Mutation
EZH2: Y641N
Host Species/Expression System
Sf9
Purity

≥90%

Format
Aqueous buffer solution
Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable FLAG peptide, and 20% glycerol

MW
EZH2: 87 kDa; EED: 51 kDa; SUZ12: 88 kDa; AEBP2: 54 kDa; RbAp48: 48 kDa
Amino Acids
EZH2: 2-746(end); EED: 2-441(end); SUZ12: 1-739(end); RbAp48: 1-503(end); AEBP2: 2-425(end)
Genbank #
EZH2: NM_004456; EED: NM_003797, SUZ12: NM_015355; AEBP2: NM_153207; RbAp48: NM_005610
UniProt #
EZH2: Q15910; EED: O75530; SUZ12: Q15022; RbAp48: Q09028; AEBP2: Q6ZN18
Tag(s)
EZH2: N-terminal His-tag; EED: N-terminal FLAG-tag; SUZ12: N-terminal His-tag; RbAp48: N-terminal His-tag; AEBP2: N-terminal His-tag
Background

EZH2 (enhancer of zeste homolog 2) is the catalytic subunit of the PRC2 (polycomb repressive complex 2)/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. It is abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

References

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Application Reference:
Morin, R.D., et al. Nat Genet. 2010 February; 42(2): 181–185.