EZH2 (non-complexed), His-GST-tags

Catalog #: 50279
Size: 20 µg
Species: Human
Host Species: Sf9 insect cells
MW: 114 kDa
Genbank #: NM_004456
Tag(s): N-terminal His-GST-tags
a.a: 2–end
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-GST-tag, MW= 114 kDa, expressed in a Sf9 baculovirus expression system. This protein is inactive alone, although it is functional if combined with other proteins in the complex.
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH, KMT6
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol.
Aqueous buffer solution
Storage / Stability:

≥ 6 months at -80°C

Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Application Reference(s):
1. EZH2 contributes to the response to PARP inhibitors through its PARP-mediated poly-ADP ribosylation in breast cancer (2017)

Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Recombinant Protein
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