EZH2 (non-complexed), His-GST-tags Recombinant

Catalog #

50279

$490 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit), encompassing amino acids 2-751 (end). The construct contains an N-terminal His-tag (6xHis) followed by a GST tag, linked to the protein via a short peptide linker corresponding to a thrombin cleavage site. The recombinant protein was affinity purified.

This product has been cited 5 times.

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Synonyms

ENX-1, KMT6, Lysine N-methyltransferase 6

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

EZH2-EED inhibition curve using an AlphaLISA assay (#52066). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations5

Species

Human

Construct

EZH2 (His-GST-2-751(end))

Host Species/Expression System

Sf9

Purity

≥81%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 240 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% Glycerol, 3 mM DTT

114 kDa

Amino Acids

2-751(end)

Genbank #

NM_004456

UniProt #

Q15910

Tag(s)

N-terminal His-GST-tags

Background

EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

References

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

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Datasheets/Protocols

50279 Lot 201109-G

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EZH2 (non-complexed), His-GST-tags Recombinant

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