EZH2-EED Binding Assay Kit

Catalog #

52066

$1,665 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The EZH2-EED Binding Assay Kit is designed to measure the inhibition of the protein-protein interaction between EZH2 and EED. The key to this kit is the specific binding of EZH2 to EED. With this kit, only two simple steps on a microtiter plate are required. First, incubate a sample containing EZH2, EED and an inhibitor of choice. Next, incubate with a mixture of acceptor and donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against EZH2-EED? Check out our Methyltransferase Screening Services.

This product has been cited 1 time.

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Synonyms

KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH, EED

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

AlphaLISA®

Supplied As

The kit comes in a convenient AlphaLISA® format, with GST-tagged EZH2, FLAG-tagged EED, and enough assay buffer to perform a total of 384 reactions.

Format

Catalog Number	Component	Amount	Storage
50279	EZH2, GST-tag	20 μg	-80°C	Avoid freeze/ thaw cycles!
50280	EED, FLAG-tag	10 μl	-80°C
52170-B	4x HMT Assay Buffer 2B	4 ml	-20°C

UniProt #

Q15910

Background

EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27

References

Kong, X., et al., J. Med. Chem. 2014; 57:9512.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Assay Conditions

Assay was done using AlphaLISA format to measure the inhibition of the protein-protein interaction between EZH2 and EED. Reaction was performed at room temperature for 1 hour. Anti-FLAG Acceptor Beads and Glutathione Donor Beads were added and the reaction was incubated for 1 hour at room temperature. Alpha Counts were read on AlphaScreen microplate reader.

Instructions for Use

See product datasheet for detailed assay protocol.

Applications

Useful for the study of EZH2 binding assays, screening inhibitors and selectivity profiling.

Shipping Temperature

Dry ice

Notes

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Anti-FLAG AlphaLISA® Acceptor Beads Beads, 5 mg/ml (PerkinElmer #AL112C)
AlphaScreen® Glutathione Donor Beads, 5 mg/ml (PerkinElmer #6765300)
Optiplate-384 (PerkinElmer #6007290) AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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EZH2-EED Binding Assay Kit

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