EZH2 Chemiluminescent Assay Kit

Catalog #: 52009L
Size: 96 reactions
$1,425
Background:
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Description:
The EZH2 Direct Activity Chemiluminescent Assay Kit is designed to measure activity of the EZH2 complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling applications. The key to the EZH2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, reagents, and purified EZH2 complex for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Dillon S.C., et al. 2005. Genome Biology 6:227.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
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Product Datasheets

52009L.pdf