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Description
Mutant version of BPS regular EZH2 5-member complex (Cat. # 51004), but with a Ala-to-Gly mutation on a.a. 682 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, A682G, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
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Synonyms
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH2 (A682G), EZH, Retinoblastoma Binding Protein 4 (RBBP4)
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Product Data Gallery
4-20% Gradient SDS-PAGE Coomassie Staining
EZH2 (A677G) inhibition curve using a chemiluminescent assay (#52077). Representative data only; data may vary by lot.
Product Info
Storage and Usage
Citations
Host Species/Expression System
Sf9 insect cells
Amino Acids
2–end (all components)
Genbank #
NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Background
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
References
1. Varambally, S. et al. Science 322
(5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32
(2008).
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipping Temperature
-80°C
Notes
This mutation is equivalent to A677G in variant 3 (GenBank Accession No. NM_001203247).
Warnings
Avoid freeze/thaw cycles
Scientific Category
Methyltransferase
Regulatory Status
Research Use Only
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