Human/Mouse ERK2 (K52R), Inactive, His-tag

Catalog #: 40056
Size: 100 µg
$360
Species: Mouse
Host Species: E. coli
MW: 42 kDa
Genbank #: NM_0011949
Tag(s): N-terminal His-tag
a.a: full length
Description:
Human/Mouse ERK2/MAPK2 (K52R) (GenBank Accession No. NM_0011949), full length with mutation on lysine52 to arginine and with an N-terminal His-tag, MW=42 kDa, expressed in E. coli . The K52R mutation prevents ATP binding, making the kinase inactive.
Synonym(s):
ERK2, MAPK1, mitogen-activated protein kinase 1, Extracellular Signal-Regulated Kinase 2, PRKM
Specific Activity:
inactive
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 125 mM imidazole, 3 mM DTT, 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful as a substrate for MEK1.
Reference(s):

1. Robinson, M.J., et al. Biochemistry. 1996 May 7;35(18):5641-6.
2. Mitsushima, M. et al. Cell Res. 313 (9), 1830- 1838 (2007).
3. Wille, L. et al. Mol. Immunol. 44 (12), 3085- 3091 (2007).

Application Reference(s):
1. BRAF-inhibitor Associated MEK Mutations Increase RAF-Dependent and -Independent Enzymatic Activity (2017)

Notes:
The human and mouse versions of this protein share 100% amino acid homology.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Substrate
Product Type:
Recombinant Protein
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Product Datasheets

40056_Lot100708.pdf