SMARCA2, GST-tag

Catalog #: 31141
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 43 kDa
Genbank #: NM_003070
Tag(s): N-terminal GST-tag
a.a: 1375–1511
Description:
Human SMARCA2, also known as BRM, GenBank Accession No. NM_003070, a.a. 1375-1511 corresponding to single bromodomain, with N-terminal GST-tag, MW=43kDa, expressed in expressed in an E. coli expression system.
UniProt
P51531
Synonym(s):
SMARCA2, BRM, BRG1-Associated Factor 190B, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin 2, BAF190, SNF2L2
Assay Conditions:

SMARCA2 is incubated with Bromodomain Ligand 2 in SMARCA2 buffer in a 10 μl reaction for 30 minutes. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer). After 10 minutes incubation at RT, Alphacounts are measured.

Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
1. Van Houdt JK., et al. Nat Genet. 2012; 44(4):445-9.
2. Wolff D., et al. Mol Syndromol. 2012; 2(6):237-244.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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Product Datasheets

31141_Lot130614-D.pdf