PRMT5/MEP50, FLAG-tag, His-tag (HEK293-derived)

Catalog #: 51045
Size: 20 µg
$435
Species: Human
Host Species: FreeStyle 293-F cells
MW: 73 kDa (PRMT5), 37.5 kDa (MEP50)
Genbank #: NM_006109 (PRMT5), NM_024102 (MEP50)
Tag(s): N-terminal FLAG-tag (PRMT5), N-terminal His-tag (MEP50)
a.a: 2–end (both)
Background:

Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer

Description:
Complex of human PRMT5 protein(GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.
UniProt
O14744
Synonym(s):
PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT Protein
Assay Conditions:
50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 µg/ml Flag peptide, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009).
2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008).

Application Reference(s):
Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2 (2015)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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