JARID1B (KDM5B, PLU-1), His-FLAG-tags

Catalog #: 50121
Size: 20 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 88 kDa
Genbank #: NM_006618
Tag(s): N-terminal His-FLAG-tags
a.a: 2–751
Background:
Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Description:
Human JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_006618), a.a. 2-751 with N-terminal His-FLAG-tag, MW=88 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
Q9UGL1
Synonym(s):
JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, CT31, Cancer/Testis Antigen 31, PLU-1, Jumonji, AT Rich Interactive Domain 1B, Lysine-Specific Demethylase 5B
Specific Activity:
0.1 pmole/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM a-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1B (20 – 100 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Yamane K, et al. (2007). Mol. Cell 25(6): 801–12.
2. Barrett A, et al. (2007). Int. J. Cancer 121(2): 265–75.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50121_Lot150812-1.pdf