JARID1A (KDM5A), His-Avi-tags, FLAG-tag

Catalog #: 50155
Size: 20 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 128 kDa
Genbank #: NM_001042603
Tag(s): N-terminal His-Avi-tags, C-terminal FLAG-tag
a.a: 2–1090
Description:

Human JARID1A protein, also known as KDM5A and RBBP2, GenBank Accession No. NM_001042603, a.a. 2-1090 with N-terminal His-Avi-tags and C-terminal FLAG-tag, MW= 128 kDa, expressed in Sf9 cells via a baculovirus expression system.

UniProt
P29375
Synonym(s):
KDM5A, RBBP2, JARID1A
Specific Activity:
≥0.035 pmol/min/µg
Assay Conditions:
10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM alpha-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and Jarid1A (20 – 100ng) added to the wells. Add antibody against di-methylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 µg/ml FLAG peptide, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of JARID1A enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Roesch, A., et al. (2005). Mod Pathol. 18(9):1249-57.
2. Zhou X., et al. (2010). Cancer Res. 70(10):4214-21
3. van Zutven LJ, et al. (2006). Genes Chromosomes Cancer 45(5):437-46.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50155_Lot_170217-1.pdf

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