HDAC11, GST-tag

Catalog #: 50011
Size: 50 µg
$410
Species: Human
Host Species: Sf9 insect cells
MW: 66 kDa
Genbank #: NM_024827
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human HDAC11 , full length, GenBank Accession No. NM_024827, with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus expression system.
UniProt
Q96DB2
Synonym(s):
histone deacetylase 11, HDAC-11
Specific Activity:
≥3.7 pmol/min/µg.
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC11 (15.6 - 2000 ng). Incubation condition: 35 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application Reference:
Reassessing the effects of histone deacetylase inhibitors on hippocampal memory and cognitive aging (2014)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50011_Lot150224.pdf