HDAC1, FLAG-tag, His-tag

Catalog #: 50051
Size: 50 µg
Species: Human
Host Species: Sf9 insect cells
MW: 56 kDa
Genbank #: NM_004964
Tag(s): C-terminal FLAG-tag, C-terminal His-tag
a.a: full length
Human HDAC1 protein (GenBank Accession No. NM_004964), full length with C-terminal His-tag and C-terminal FLAG-tag, MW = 56 kDa, expressed in baculovirus expression system. Recombinant HDAC1 forms a complex with endogeneous Hsp70 and is co-purified with tubulin. The identity of Hsp70 and tubulin was confirmed by MALDITOF mass spectrometry.
histone deacetylase 1, HDAC-1, HDAC1 Tag
Specific Activity:
≥460 pmol/min/µg.
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC1. HDAC reaction was done for 30 min at 37°C followed by treatment with 50 µl of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rikimaru,T. et al., Oncology 72 (1-2), 69-74 (2007).
2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

Application References:
1. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases (2015)
2. A chimeric SERM-histone deacetylase inhibitor approach to breast cancer therapy (2014)
3. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis (2014)
4. Design, synthesis and evaluation of novel HDAC inhibitors as potential antitumor agents (2014)
5. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
6. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
7. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
8. Kinetic method for the large-scale analysis of the binding mechanism of histone deacetylase inhibitors (2014)
9. Selective HDAC Inhibition for the Disruption of Latent HIV-1 Infection (2014)
10. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
11. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014)
12. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
13. Class I HDAC imaging using [ (3)H]CI-994 autoradiography (2013)
14. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
15. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
16. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
17. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹⁸F-FAHA (2013)
18. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni (2013)
19. Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors (2013)

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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Product Datasheets

50051 Lot 160519.pdf