eIF4E, GST-tag

Catalog #: 40530
Size: 100 µg
$415
Species: Human
Host Species: E. coli
MW: 51 kDa
Genbank #: NM_001968
Tag(s): N-terminal GST-tag
a.a: 2–216
Description:
Human eIF4e (eukaryotic translation initiation factor 4E) (GenBank Accession No. NM_001968), a.a. 2-216 with N-terminal GST-tag, MW= 51 kDa, expressed in an E. coli expression system.
UniProt
P06730
Synonym(s):
EIF4E1, Eukaryotic Translation Initiation Factor 4E, mRNA Cap-Binding Protein, EIF-4E
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Suitable substrate for MNK1 kinase and other kinase assays.
Reference(s):

1. Shih,J.W. et al., Oncogene 27(5), 700-714 (2008).
2. Salehi Z., Mashayekhi F. and Shahosseini F. Cell Biol. Int. 31(11), 1400-1404 (2007).

Application Reference:
MNK1-induced eIF-4E phosphorylation in myeloma cells: a pathway mediating IL-6-induced expansion and expression of genes involved in metabolic and proteotoxic responses (2014)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Substrate
Product Type:
Recombinant Protein
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