EHMT1 (GLP) Chemiluminescent Assay Kit

Catalog #
53007
$750 *
Size: 96 reactions
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Description

The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure EHMT1 (also known as G9a-like protein) activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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This product has been cited 1 time.

Synonyms
EHMT1, GLP, KMT1D, G9a-like protein, euchromatic histone-lysine N-methyltransferase
Product Info
Storage and Usage
Citations1
Assay Kit Format
Chemiluminescent
Supplied As
The EHMT1 (GLP) Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, primary antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EHMT1 for 96 enzyme reactions.
Format
Catalog # Component Amount Storage
51020 EHMT1 (GLP) 4 µg -80°C Avoid freeze/thaw cycles!
52140E Primary antibody 5 12.5 µl -80°C
52130H Secondary HRP-labeled antibody 1 10 µl -80°C
52120 20 µM S-adenosylmethionine 250 µl -80°C
52160 4x HMT assay buffer 1* 3 ml -20°C
79556 Blocking buffer 1 50 ml +4°C
79670 ELISA ECL substrate (2 components) 6 ml each Room Temp.
  96-well plate precoated with histone substrate 1 plate +4°C

*Add 125 µl of 0.5M DTT.

UniProt #
Q9H9B1
References
Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.