EGFR (T790M, L858R), His-tag, GST-tag

Catalog #: 40350
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 88 kDa
Genbank #: NM_005228
Tag(s): C-terminal His tag, N-terminal GST-tag
a.a: 668–1210
Description:
Human EGFR T790M L858R (GenBank Accession No. NM_005228), a.a. 668-1210 with substitutions of Threonine790 to Methionine and Leucine858 to Arginine and a C-terminal His-tag and N-terminal GST-tag, MW= 88 kDa, expressed in a baculovirus expression system.
UniProt
P00533
Synonym(s):
EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Specific Activity:
1,200 pmol/min/μg.
Assay Conditions:
Assay was performed in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
50 mM Tris-HCl, pH 8.5, 250 mM NaCl, 10% glycerol and 2 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007)
2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007).

Application Reference:
Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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Product Datasheets

40350_Lot_171010-1.pdf