EGFR (T790M/C797S/ L858R) Kinase Assay Kit

Catalog #

40326

$535 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. T790M, L858R and C797S mutations in EGFR cause resistance to known EGFR inhibitors. The EGFR(T790M/C797S/L858R) Kinase Assay Kit is designed to identify mutant-selective EGFR kinase inhibitors and to measure EGFR (T790M/C797S/L858R) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against EGFR (T790M/C797S/ L858R)? Check out our Kinase Screening Services.

●

Synonyms

EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The EGFR (T790M/C797S/L858R) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M/C797S/L858R) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40351	EGFR (T790M/C797S/L858R)	2 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40217	PTK substrate Poly (Glu:Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P00533

References

Wang, S., et al. J. Hematology & Oncology 9:59 (2016)

Jia, Y., et al. Nature 534(7605): 129–132 (2016).

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only