EGFR (A750P), DEL 747-749, GST-Tag Recombinant

Catalog #
79437
$410 *
Size: 10 µg
Qty
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Description

Recombinant Human EGFR also known as ERBB, mENA, ERBB1, and HER1 with A750P mutations and 747-749 deletion. Genbank Accession NM_005228, a.a. 695 end, with an N-terminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 87 kDa.

Synonyms
ERBB, mENA, ERBB1, HER1
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells using baculovirus
Purity

≥70% 

Formulation
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
MW
87 kDa
Genbank #
NM_005228
UniProt #
P00533
Tag(s)
N-terminal GST-Tag
Background
EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.
References

1. Wang, K., et al., J Biol. Chem 2004;279:53848-53856.

2. Kobayashi, S., et al., New Eng. J. Med. 2005;325:786-792.