EGFR, His-tag, GST-tag

Catalog #: 40187
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 87.3 kDa
Genbank #: NM_005228
Tag(s): C-terminal His-tag, N-terminal GST-tag
a.a: 668 –1210
Description:
Human EGFR (GenBank Accession No. NM_005228), (a.a. 668-1210) with C-terminal His-tag and N-terminal GST tag, MW=87.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Specific Activity:
≥800 pmol/min/μg.
Assay Conditions:
Assay was performed in a kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 30 min. ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol and 1 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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Product Datasheets

40187_Lot120321-GC_R.pdf