EGFR, His-tag, GST-tag Recombinant

Catalog #

40187

$395 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human EGFR (epidermal growth factor receptor), encompassing amino acids 668-1210(end). This construct contains an N-terminal GST-tag and a C-terminal His tag (6xHis). The recombinant protein was affinity purified.

This product has been cited 20 times.

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Synonyms

EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations20

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT.

88 kDa

Amino Acids

668 –1210 (end)

Specific Activity

800 pmol/min/μg

Genbank #

NM_005228

UniProt #

P00533

Tag(s)

C-terminal His-tag, N-terminal GST-tag

References

1. Nakamura, J.L. Expert Opin. Ther. Targets 11 (4), 463-472 (2007).
2. Kim, H.J., et al. Curr. Biol. 17 (5), 455-461 (2007).

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 minutes. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40187 Lot 200116-2

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EGFR, His-tag, GST-tag Recombinant

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