DOT1L (KMT4) Chemiluminescent Assay Kit

Catalog #: 52202
Size: 96 reactions
$695
Description:
The DOT1L Chemiluminescent Assay Kit is designed to measure DOT1L activity for screening and profiling applications. The key to the DOT1L Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
KMT4, KIAA1814
Supplied As:
The DOT1L Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate, DOT1L substrate, primary antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
One year from date of receipt when stored as directed.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
1. Min, J., et al. Cell 2003; 112:711-723.
2. Daigle, S.R., et al. Cancer Cell 2011, 20:53-65.

Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

52202.pdf