DNMT3B Chemiluminescent Assay Kit

Catalog #: 52034
Size: 96 reactions
The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT3B/3L complex for 100 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and HTS applications.
Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
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