DNMT3A Chemiluminescent Assay Kit

Catalog #

52033

$875 *

Size: 96 reactions

Qty

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Description

The DNMT3A Direct Activity Assay Kit is designed to measure DNMT3A activity for screening and profiling applications. The key to the DNMT3A Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3A activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3A/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against DNMT3A? Check out our Methyltransferase Screening Services.

This product has been cited 3 times.

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Synonyms

DNMT-3A

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Product Data Gallery

Product Info

Storage and Usage

Citations3

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well plate precoated with DNA substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT3A/3L complex for 100 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
51106	DNMT3A/3L Complex	20 µg	-80°C	Avoid freeze/ thaw cycles!
52120	400 µM S-adenosylmethionine	2 x 250 µ	-80°C
	Anti-5-methylcytosine antibody	25 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
52201	4x DNMT assay buffer 2*	5 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate (2 components)	6 ml each	+4°C
	Plate pre-coated with DNA substrate	1	+4°C

UniProt #

Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)

References

Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for screening enzyme inhibitors.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only