DNMT1 Chemiluminescent Assay Kit

Catalog #: 52050L
Size: 96 reactions
$805
Description:
The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
TRDMT1
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, the antibody against 5-methylcytosine, the secondary HRP-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Useful for studying enzyme kinetics and HTS applications.
Reference(s):

Svedruzic, Z.M., Curr Med Chem. 2008;15(1):92-106.

Application Reference(s):
ATM mediates pRB function to control DNMT1 protein stability and DNA methylation (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
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Product Datasheets

52050L.pdf