DNMT1 Chemiluminescent Assay Kit

Catalog #

52050L

$865 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against DNMT1? Check out our Methyltransferase Screening Services.

This product has been cited 4 times.

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Synonyms

TRDMT1, DNA, DNMT-1

Product Info

Storage and Usage

Citations4

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well plate precoated with DNA substrate, the antibody against 5-methylcytosine, the secondary HRP-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
0.5M DT

Format

Catalog Number	Component	Amount	Storage
51101	DNMT1	2 x 10 μg	-80°C	Avoid freeze/ thaw cycles!
52120	400 μM S-adenosylmethionine	2 x 250 μl	-80°C
	Anti-5-methylcytosine antibody	25 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
52200	4x DNMT assay buffer 1* *Add 260 μl of 0.5 M DTT before use.	5 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate A (translucent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	Plate precoated with DNMT1 substrate	1	+4°C

UniProt #

P26358

References

Svedruzic, Z.M., Curr Med Chem. 2008;15(1):92-106.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for screening enzyme inhibitors.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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DNMT1 Chemiluminescent Assay Kit

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