DNA Polymerase γ (POLG), His-FLAG-Tag Recombinant

Catalog #

21001

$350 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human DNA polymerase γ (gamma), full length encompassing amino acids 2-1239 (end). This construct contains an N-terminal His-tag (6xHis) followed by a FLAG-tag. The recombinant protein was affinity purified.

This product has been cited 2 times.

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Synonyms

DNA polymerase subunit gamma-1, Pol gamma, POLG, PEO, Mitochondrial DNA polymerase catalytic subunit, MDP1, SCAE, MIRAS, POLG1, PolG-alpha, POLGA, SANDO, MTDPS4A, MTDPS4B

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

POLG Activity

DNA Polymerase γ inhibition curves using a fluorogenic assay (#82098). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

DNA Polymerase γ (His-FLAG-2-1239(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 µg/ml FLAG peptide

141 kDa

Amino Acids

2-1239(end)

Genbank #

NM_002693

UniProt #

P54098

Tag(s)

N-terminal His-FLAG-tag

Background

Polymerases are the enzymes responsible for synthesizing nucleic acids. Polymerase γ, also known as DNA polymerase subunit gamma, POLG or POLG1, belongs to the Family A of DNA polymerases and corresponds to the catalytic subunit of the mitochondrial DNA polymerase. It works in conjunction with POLG2 to replicate mitochondrial DNA. In addition to its DNA polymerase activity, it has a 3’-5’ exonuclease and a 5’dRP lyase activity for proofreading and repair. Mutations in polymerase γ cause mitochondrial dysfunction, as observed in SANDO (sensory ataxia neuropathy dysarthia and ophthalmoparesis), AHS (Alpers-Huttenlocher Syndrome) and MNGIE (mitochondrial neurogastrointestinal encephalopathy syndrome). Alternatively, the inhibition of DNA synthesis can be used as a therapeutic approach for diseases in which cell division or energetic needs are uncontrolled, such as cancer. Further studies into DNA polymerase inhibitors will bring new therapy options to cancer patients.

References

1. Ropp, P.A., and Copeland, W.C., Genomics 1996;36(3):449-458.
2. Lestienne, P., Biochem. Biophys. Res. Comm. 1987;146(3): 1146-1153.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed using Human DNA Polymerase Assay Kit (ProFoldin #DPB100K) and with various amounts of DNA Polymerase γ enzyme. The assay was run in 384-well format with a reaction volume of 15 µl supplemented with 15 µl of detection reagent (total 30 µl) prior plate reading. Plate was incubated for 1 hour at 37°C prior detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Molecular Biology

Regulatory Status

Research Use Only