DNA Polymerase γ (POLG), His-FLAG-tag

Catalog #: 21001
Size: 100 µg
$340
Species: Human
Host Species: Sf9 insect cells
MW: 141 kDa
Genbank #: NM_002693
Tag(s): N-terminal His-FLAG-tag
a.a: 2–1239 (end)
Background:
POLG is the catalytic subunit of the mitochondrial DNA polymerase. The catalytic subunit contains three enzymatic activities, a DNA polymerase activity, a 3’-5’ exonuclease activity that proofreads misincorporated nucleotides, and a 5’-dRP lyase activity required for base excision repair. Mutations in the POLG gene are associated with several mitochondrial diseases, including Alpers' disease, ataxia-neuropathy disorders, and dominant and recessive forms of progressive external ophthalmoplegia.
Description:
Human DNA polymerase γ (gamma), also known as Pol gamma and POLG (GenBank Accession No. NM_002693), a.a. 2-1239 (end), with N-terminal His-tag and N-terminal FLAG-tag, MW=141 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Synonym(s):
DNA polymerase gamma, Pol gamma, POLG
Formulation:

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 0.04% Tween-20, and 20% glycerol.

Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for DNA synthesis and DNA repair, as an antigen for Western blotting, and for inhibitor screening.
Reference(s):

1. Ropp, P.A., and Copeland, W.C., Genomics 1996;36(3):449-458.
2. Lestienne, P., Biochem. Biophys. Res. Comm. 1987;146(3): 1146-1153.

Application Reference(s):
1. Identification of mechanistically distinct inhibitors of HIV-1 reverse transcriptase through fragment screening (2015)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Molecular Biology
Product Type:
Recombinant Protein
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