DEP1(PTPRJ), GST-tag

Catalog #: 30050
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 58.9 kDa
Genbank #: NM_002843
Tag(s): N-terminal GST-tag
a.a: 1024–1306
Background:
Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Positive regulator of platelet activation and thrombosis. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation through KDR dephosphorylation. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration through dephosphorylation of PDGFR. Negative regulator of EGFR signaling pathway via EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
Description:
Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), also known as PTPRJ, PTP-eta and CD148 (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system.
Synonym(s):
DEP1, density-enhanced phosphatase-1, PTP-eta, CD148, PTPRJ
Specific Activity:
268 nmole/min/µg
Assay Conditions:
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30&Deg;C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12.
2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Receptor
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

30050_Lot150226.pdf