Den1, GST-tag

Catalog #: 81084
Size: 25 µg
$305
Species: Human
Host Species: E. coli
MW: 51 kDa
Tag(s): GST-tag
Background:
DEN1 (SENP8, NEDP1) is a deNEDDylase that deconjugates NEDD8 conjugates. A recent study demonstrated that DEN1 deNEDDylated the tumor suppressor breast cancer-associated protein BCA3. Full length DEN1 cleaves NEDD8-PLA2 with high efficiency.
Description:
Human Den1 recombinant protein, with GST-tag, expressed in E. coli.
UniProt
Q96LD8
Synonym(s):
DEN1, Deneddylase-1, FKSG8, HsT17512, NEDD8-specific protease 1, NEDP1, Protease, cysteine 2, PRSC2, Sentrin/SUMO-specific protease SENP8, Sentrin-specific protease 8
Format:
Aqueous buffer solution
Storage / Stability:

Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.

Application(s):
This enzyme is active in the NEDD8-IsoPro assay. Suggested Substrate: NEDD8-AMC
Reference(s):
1. Mendoza, H.M., et al., NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins. J. Biol. Chem., 2003. 278(28):25637?43.
2. Gan?Erdene, T., et al., Identification and characterization of DEN1, a deneddylase of the ULP family. J. Biol. Chem., 2003. 278(31):.28892?900.
3. Wu, K., et al., DEN1 Is a Dual Function Protease Capable of Processing the C-Terminus of Nedd8 and Deconjugating Hyper?neddylated CUL1. J. Biol. Chem., 2003. 278(31):28882?28891.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (DeNEDDylase)
Product Type:
Recombinant Protein
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