Demethylase(s)

 

Demethylases are enzymes that remove methyl groups from lysine and arginine residues of proteins, the most commonly studied being histone demethylases. Methylation of histones is essential for regulating transcription and maintaining chromatin structure, and demethylases, like all histone modifying enzymes, play an important role in development and gene regulation. All of the demethylases, with the exception of LSD1, contain a catalytic jumonji domain. Many of these enzymes require a cofactor such as FAD, Fe(II), or α-ketoglutarate.

Like, methyltransferases, demethylases exhibit a high level of substrate specificity with respect to their target lysine. In addition, they are sensitive to the specific type of methylation that they recognize and subsequently demethylate. For example, some demethylases are only capable of demethylating mono- and dimethyl substrates, whereas others are capable of demethylating all states of lysine methylation.

Given the critical role that demethylases play in gene regulation, it is not surprising that they have been linked to many human cancers. BPS was the first company to supply active, high purity histone demethylases for life science research. We provide a unique and comprehensive offering of enzymes, assay kits, and inhibitors/activators to meet all of your research needs. Our large portfolio of substrates, which includes and a number of modified histones, makes it easy to study histone demethylase substrate specificity.

References:

1.  Pedersen, M.T., et al., Trends Cell Biol. 2010; 20(11): 662-671.

 

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Catalog#SizeProduct NamePrice
50613 384 reactions LSD2(KDM1B) Homogeneous Assay Kit (Monomethyl)
$995
50122 20 µg Mouse JARID1B (KDM5B, PLU-1), FLAG-tag
$435
27635-2 25 mg OG-L002
$490
27635-1 5 mg OG-L002
$165
50515 384 reactions PHF8 (KDM7B) Homogeneous Assay Kit
$1,950
50131 20 μg PHF8 (KDM7B), (2-525), FLAG-tag
$450
50132 20 μg PHF8 (KDM7B), Full-length, FLAG-tag
$450
27639-1 5 mg Posaconazole
$140
27639-2 25 mg Posaconazole
$490
52131H 10 µl Secondary Anti-HRP-labeled Antibody 2
$120
50651 96 reactions TET1 Chemiluminescent Assay Kit
$680
50161 50 µg TET1, FLAG-tag
$445
50652 96 reactions TET2 Chemiluminescent Assay Kit
$680
50162 50 ug TET2, FLAG-His-tag
$445
50163 50 µg TET3, FLAG-Tag, His-Tag
$445
27305 100 mg Tranylcypromine (2-PCPA)
$60
50116 20 µg UTX (KDM6A), C-term FLAG-tag
$435
50119 20 μg UTX (KDM6A), N-terminal FLAG-tag
$460
50615 96 reactions UTX Chemiluminescent Assay Kit
$660
50516 384 reactions UTX(KDM6A) Homogeneous Assay Kit
$1,950

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