Histone Octamer H3K27cMe2, His-tag

Catalog #: 52090
Size: 50 µg
$425
Species: Human
Host Species: E. coli
MW: 116 kDa
Genbank #: NM_033445, NM_003528, NM_003532, and NM_003548
Tag(s): N-terminal His-tag
Background:
The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification
Description:
Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, dimethyl aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.
UniProt
P68431
Formulation:
45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
A perfect substrate for histone methyltransferases and demethylases.
Reference(s):
1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase, Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

52090_Lot140214.pdf