Histone H3-H4 Tetramer H3k27cMe3, His-tag

Catalog #: 52087
Size: 50 µg
$425
Species: Human
Host Species: E. coli
MW: 57 kDa
Genbank #: NM_033445, NM_003528, NM_003532, and NM_003548
Tag(s): N-terminal His-tag
Description:
Human recombinant histone tetramer consisting of 2 molecules each of histones H3 (a.a. 2-136(end)) and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, trimethyl aminoethylcysteine. GenBank Accession Nos. NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal Histag. Global MW = 57 kDa.
UniProt
P68431
Synonym(s):
H3/H4
Formulation:
45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
A perfect substrate for histone methyltransferases and demethylases.
Reference(s):
1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

52087_Lot140214.pdf