The UTX Homogeneous Assay Kit is designed to measure the activity of UTX for screening and profiling applications. The key to the UTX Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, UTX enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Need us to run inhibitor screens or profile your compounds against UTX (KDM6A)? Check out our Demethylase Screening Services.
The UTX Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified UTX for 384 enzyme reactions.
UTX, also known as KDM6A, is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3
References
1.Greenfield A, et al. 1998. Hum. Mol. Genet.7(4): 737–42. 2. Agger K, et al. 2007. Nature 449(7163): 731–4.
Storage/Stability
At least 6 months from date of receipt when stored as directed.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and HTS applications.
Shipping Temperature
-80°C (dry ice)
Notes
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Contraindications
Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
Warnings
Avoid freeze/thaw cycles. This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous. Do not ingest, inhale, get in eyes, on skin, or on clothing. If so, wash thoroughly.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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