UTX Chemiluminescent Assay Kit

Catalog #

50615

$715 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The UTX Chemiluminescent Assay Kit is designed to measure UTX activity for screening and profiling applications. UTX, also known as KDM6A, is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 on histone H3 (H3K27me2/3). The key to the UTX Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, UTX enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

This product has been cited 1 time.

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

Chemiluminescent

Supplied As

The UTX Chemiluminescent Assay Kit comes in a convenient 96-well strip plate format, precoated with methylated histone H3 peptide substrate, primary antibody, HRP-labeled secondary antibody, demethylase assay buffer, and purified UTX for 96 enzyme reactio

Format

Catalog #	Component	Amount	Storage
50119	UTX (KDM6A), N-term FLAG-tag	2.5 µg	-80°C
52140F	Primary antibody 6	12.5 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
	4x UTX direct assay buffer	3 x 1 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	8-well strip plate module precoated with histone substrate	1	+4°C

UniProt #

O15550

References

Greenfield A, et al. 1998. Hum. Mol. Genet. 7(4): 737–42.

Agger K, et al. 2007. Nature 449(7163): 731–4.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only