JMJD1A Homogeneous Assay Kit
The JMJD1A Homogeneous Assay Kit is designed to measure JMJD1A activity for screening and profiling applications. JMJD1A, also known as JHDM2A and KDM3A, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K9Me2 and H3-K9Me1. The JMJD1A Homogeneous The key to the JMJD1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Scheme 1: Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.
Need us to run inhibitor screens or profile your compounds against JMJD1A (KDM3A)? Check out our Demethylase Screening Services.
AlphaLISA® anti-RIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate -96 (PerkinElmer #6005290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
96 Reactions:
Catalog |
Component |
Amount |
Storage |
|
5 µg |
-80°C |
|
||
52140Q4 |
Primary antibody 17-4 |
5 µl |
-80°C |
|
79845 |
Biotinylated histone H3 peptide substrate |
300 rxns |
-80°C |
|
3 ml |
-80°C |
|||
2 ml |
-20°C |
384 Reactions:
Catalog |
Component |
Amount |
Storage |
|
20 µg |
-80°C |
|
||
52140Q4 |
Primary antibody 17-4 |
20 µl |
-80°C |
|
79845 |
Biotinylated histone H3 peptide substrate |
500 rxns |
-80°C |
|
3 ml |
-80°C |
|||
2 ml |
-20°C |
Horton, J.R., et al. Nature Structural & Molecular Biology 2010; 17(1): 38-43.