JMJD1A Homogeneous Assay Kit

Only %1 left

Catalog #

50412

As low as $850 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The JMJD1A Homogeneous Assay Kit is designed to measure JMJD1A activity for screening and profiling applications. JMJD1A, also known as JHDM2A and KDM3A, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me² and H3-K₉Me¹. The JMJD1A Homogeneous The key to the JMJD1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

50414 scheme 1

Scheme 1: Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.

Need us to run inhibitor screens or profile your compounds against JMJD1A (KDM3A)? Check out our Demethylase Screening Services.

●

Synonyms

JMJD1A, KDM3A, JHDM2A

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD1A for 96 or 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA^® anti-RIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)
AlphaScreen^® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate -96 (PerkinElmer #6005290)
AlphaScreen^® microplate reader
Adjustable micropipettor and sterile tips

Format

96 Reactions:

Catalog Number	Component	Amount	Storage
50130	JMJD1A (KDM3A)	5 µg	-80°C	Avoid freeze/ thaw *cycles!*
52140Q4	Primary antibody 17-4	5 µl	-80°C
79845	Biotinylated histone H3 peptide substrate	300 rxns	-80°C
52407	4x HDM Assay Buffer 2	3 ml	-80°C
52301	4x Detection buffer	2 ml	-20°C

384 Reactions:

Catalog Number	Component	Amount	Storage
50130	JMJD1A (KDM3A)	20 µg	-80°C	Avoid freeze/ thaw cycles!
52140Q4	Primary antibody 17-4	20 µl	-80°C
79845	Biotinylated histone H3 peptide substrate	500 rxns	-80°C
52407	4x HDM Assay Buffer 2	3 ml	-80°C
52301	4x Detection buffer	2 ml	-20°C

UniProt #

Q9Y4C1

References

Horton, J.R., et al. Nature Structural & Molecular Biology 2010; 17(1): 38-43.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See product datasheet for detailed instructions.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

JMJD1A Homogeneous Assay Kit

PRODUCTS

SERVICES

RESOURCES

COMPANY